癌变·畸变·突变 ›› 2006, Vol. 18 ›› Issue (5): 400-403.doi: 10.3969/j.issn.1004-616x.2006.05.020

• 技术与方法 • 上一篇    下一篇

彗星实验检测紫外线诱导的K562细胞DNA损伤

罗明志1,2;齐 浩1;陈文芳1;刘 淼1;郭 伟1   

  1. 1．陕西师范大学生命科学学院，陕西 西安　710062；2．西安文理学院生命科学系，陕西 西安 710065
  • 收稿日期:2005-05-12 修回日期:2006-03-21 出版日期:2006-09-30 发布日期:2006-09-30
  • 通讯作者: 齐 浩

DNA Damage Induced by UV in K562 Cells Measured by Comet Assay

LUO Ming-zhi1,2, QI Hao1, CHEN Wen-fang1, LIU Miao1, GUO Wei1   

  1. 1.College of Life Science, Shanxi Normal Univercity, Xi'an 710062;2.Departmemt of Life Science, Xi'an University of Art and Science，Xi'an 710065
  • Received:2005-05-12 Revised:2006-03-21 Online:2006-09-30 Published:2006-09-30
  • Contact: QI Hao

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摘要： 背景与目的：研究紫外线（Ultraviolet，UV）诱导K562细胞DNA损伤情况，评价彗星实验改良方法及分析参数的可靠性。材料与方法：采用0.3 mW/cm2 UV照射K562细胞，用不同时间（3、5、10、40、60、120、180、240 s）诱导细胞DNA损伤，用彗星实验检测，CASP软件分析。结果：尾长、彗星长、尾矩、Olive尾矩4个参数与紫外线照射时间有良好的时间依赖关系。结论：0.3 mW/cm2的紫外线照射K562细胞3 s即可造成细胞DNA损伤，CASP分析软件可以用于彗星检测分析，其中尾长、彗星长、尾矩、Olive尾矩可作为彗星实验的分析指标，建立了可靠性较强的改良彗星实验系统。

关键词: DNA损伤, 单细胞凝胶电泳, 紫外线, K562

Abstract: BACKGROUND ＆ AIM： This article exploved a modified method of comet assay from the Singh's alkaline comet assay and discussed the reliability of this method and the CASP software used to measure the DNA damage induced by UV light． MATERIAL AND METHODS： DNA damage of K562 cells induced by UV for different times (3 s,5 s,10 s,40 s,60 s,120 s,180 s,240 s) was measured by comet assay．The results were assayed by CASP． RESULTS： UV( 0.3 mW/cm2)induced a significant DNA damage (P<0.01) when the results were assayed by the four parameters (tail length，comet length，tail moment，olive tail moment)． CONCLUSION： The results suggested that the four parameters indicated a good time_dependent effect.The method which we developed and the four parameters were reliable．

Key words: DNA damage, single cell gel electrophoresis, Ultraviolet, K562

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